Dynamic NanoSIMS ion imaging of unicellular freshwater algae exposed to copper

Comparative sensitivity of freshwater algae to atrazine.

Ultra High Spatial Resolution Ion Imaging with a NanoSIMS Ion Microprobe: Applications

Introduction: Ion imaging technique with secondary ion mass spectroscopy (SIMS) is a powerful tool to visualize the distributions of isotopes and/or elements in samples, and is becoming common to variety field of sciences including material science [1], cosmochem-istry [2] and biology [3]. The CAMECA NanoSIMS 50L ion microprobe represents the in-situ microanalysis by SIMS, combining ultra high ...

Rationalizing Elemental Ratios in Unicellular Algae

In 1934, the year Harold Urey was awarded a Nobel prize in chemistry for the discovery of deuterium, Alfred Redfield (Fig. 1), a 44-year-old animal physiologist at Harvard University, proposed that marine plankton have relatively constrained elemental ratios (Redfield 1934). The so-called Redfield ratios of 106 C: 16 N: 1 P (by atoms) were subsequently embraced by many biological oceanographers...

Assessment of catalase activity, lipid peroxidation, chlorophyll-a, and growth rate in the freshwater green algae Pseudokirchneriella subcapitata exposed to copper and zinc

In this work, the effect of copper and zinc on green alga Pseudokirchneriella subcapitata was evaluated through catalase activity, lipid peroxidation by TBARS essay, growth rate, and the chlorophyll-a concentration. Catalase activity increased significantly (P < 0.05) in comparison to the control at 0.1 mg L copper and 0.075 mg L zinc, whereas the damage to the cell membrane expressed as nmols/...

Characterization of the Polycomb-Group Mark H3K27me3 in Unicellular Algae

Polycomb Group (PcG) proteins mediate chromatin repression in plants and animals by catalyzing H3K27 methylation and H2AK118/119 mono-ubiquitination through the activity of the Polycomb repressive complex 2 (PRC2) and PRC1, respectively. PcG proteins were extensively studied in higher plants, but their function and target genes in unicellular branches of the green lineage remain largely unknown...